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Image Search Results
Journal: Cell Cycle
Article Title: Curcumin induces Apaf-1-dependent, p21-mediated caspase activation and apoptosis
doi: 10.4161/cc.10.23.18292
Figure Lengend Snippet: Curcumin induces Bax-dependent, p21-mediated cytochrome c release without p53 requirement. HCT116 WT (A), HCT116-p53-/- (B), HCT116-Bax-/- (C) and HCT116-p21-/- (D) cells were treated with curcumin (15 µM) for the indicated times. Cytosolic and mitochondrial fractions were isolated and equal amounts of protein were subjected to protein gel blotting for the detection of cytochrome c (Cyt. C), cytochrome c oxidase subunit II (COX II), heat shock protein 60 (Hsp60) and actin. Actin and Hsp60 serve as loading controls. *represents a non-specific band.
Article Snippet: Primary antibodies were anti-cytochrome c (mAb, monoclonal antibody), -Apaf-1 and -
Techniques: Isolation